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Product Info Summary
| SKU: | A06206 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-WIPI1 Antibody
SKU/Catalog Number
A06206
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-WIPI1 Antibody (Catalog # A06206). Tested in ELISA, WB, IF applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
WIPI1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Cite This Product
Anti-WIPI1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06206)
Host
Rabbit
Contents
WIPI1 Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Polyclonal
Clone Number
Clone: SK7
Isotype
IgG
Immunogen
WIPI1 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human WIPI1. The immunogen is located within amino acids 350 - 400 of WIPI1.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
At least two isoforms of WIPI1 are known to exist; this antibody will detect both isoforms.
Reactive Species
A06206 is reactive to Wipi1 in Human, Mouse, Rat
Applications
A06206 is guaranteed for ELISA, IF, WB Boster Guarantee
Observed Molecular Weight
68 kDa
Calculated molecular weight
48.758kDa
Background of Wipi1
WIPI1 (WD repeat domain, phosphoinositide interacting-1), also known as WIPI1, ATG18 or WIPI49, is thought to play a role in autophagy and may regulate protein trafficking in certain recycling pathways. It contains three WD repeats and has a 7-bladed propeller structure with a conserved motif that facilitates its interaction with other proteins. WIPI1 localizes to cytoplasmic vesicles, endosomes, clathrin-coated vesicles and the trans-Golgi network. It is ubiquitously expressed with highest expression in heart, testis, placenta, pancreas and skeletal muscle. WIPI1 is upregulated in a variety of tumors, suggesting a role in carcinogenesis.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WIPI1 antibody can be used for detection of Wipi1 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in rat samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of WIPI1 in rat colon tissue lysate with WIPI1 antibody at (A) 1 and (B) 2 μg/mL.
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Immunofluorescence of WIPI1 in human colon tissue with WIPI1 antibody at 20 μg/mL.
Protein Target Info & Infographic
Gene/Protein Information For Wipi1 (Source: Uniprot.org, NCBI)
Gene Name
Wipi1
Full Name
WD repeat domain phosphoinositide-interacting protein 1
Weight
48.758kDa
Superfamily
WD repeat SVP1 family
Alternative Names
Atg18 protein homolog; Atg18; ATG18A; FLJ10055; WD repeat domain phosphoinositide-interacting protein 1; WD repeat domain, phosphoinositide interacting 1; WD40 repeat protein interacting with phosphoinositides of 49 kDa; WD40 repeat protein Interacting with phosphoInositides of 49kDa; WIPI 49 kDa; WIPI-1 alpha; WIPI-1; WIPI49ATG18
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on Wipi1, check out the Wipi1 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for Wipi1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-WIPI1 Antibody (A06206)
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No publications found for A06206
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Customer Reviews
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